Low temperature preservation of toad, Bufo, spermatozoa by Harry Leroy Barton Download PDF EPUB FB2
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Cryo-preservation of spermatozoa of the brown, brook and rainbow trout (Research contribution) [D. A Erdahl] on *FREE* shipping on qualifying : D. A Erdahl. cRYOBiOL () The Low Temperature Preservation of Boar Spermatozoa.
The Motility and Morphology of Boar Spermatozoa Frozen and Thawed in Diluent Which Contained only Sugar and Egg Yolk I. WILMUTi AND C. FOLGE C. Unit of Reproductive Physiology and Biochemistry, Huntingdon Road, Cambridge, by: 8.
Barton, H. L., and Guttman, S. ().Low temperature preservation of toad spermatozoa (genus Bufo).Tex. Sci. 23, – Beebee, T. C., and Griffiths Cited by: The average thickness of the head was shown to be μm. The total sperm length resulted to be ± μm. The average sperm concentration was estimated of × /ml.
Sperm amount, survival rate and motility were also measured. The current study was undertaken to examine the applicability of amphibian spermatozoa storage protocols developed with the cane toad (Bufo marinus) to a wider range of amphibian species, with a view to ultimately using these protocols for endangered species.
In Australia, at least 29 species of recently extinct or Low temperature preservation of toad frogs are from the. The male reproductive cycle of the toad, Bufo melanostictus, was studied with emphasis on spermatogenic activity, plasma androgen, and changes in the weights of testes, liver, and fat bodies.A total of 98 toads were collected between March and March in central Taiwan.
Histological evidence indicated that the spermatogenic cycle of this toad is of a fluctuating continuous by: The effect of monosaccharides (glucose, fructose) and disaccharides (maltose, sucrose, trehalose) as diluents, in cryoprotective additives containing Bufo (v/v) DMSO or glycerol as cryoprotectants.
Spermatozoa of the freeze-tolerant wood frog (Rana sylvatica) were used Low temperature preservation of toad develop a general protocol for the frozen storage of amphibian nce of spermatozoa to cryoprotective agents and freezing in suspension (−80°C) was determined from rates of sperm Cited by: Mean percentage and degree of motility of activated cane toad (Bufo marinus) testicular spermatozoa.
Testes were stored at 0C or 4C before release of spermatozoa by maceration. Low temperature preservation of toad spermatozoa (Genus Bufo). Tex. Storage of cane toad (Bufo marinus) sperm for 6 days at 0°C with subsequent cryopreservation.
Reprod. Fertil. Revival of spermatozoa after vitrification and dehydration at low temperatures. Nature– | Crossref Cited by: Bufo toad toxicosis is commonly recognized by veterinarians in areas where this amphibian is prevalent.
Bufo toads are distributed throughout the tropical areas of the world. The recent decline and extinction of amphibian species is a worldwide phenomenon without an identified cause or solution. Assisted reproductive techno Cited by: Storage of cane toad (Bufo marinus) sperm for 6 days at 0°C with subsequent cryopreservation.
Reprod. Fertil. Dev. 14, – | Storage of cane toad (Bufo marinus) sperm for 6 days at 0°C with subsequent by: Previous studies on cane toad (Bufo marinus; Bufonidae; Anura) sperm cryopreservation were extended to compare the effects of cryopreservation in established sucrose (non-ionic) diluents with cryopreservation in ionic diluents containing amphibian Ringer solutions (with and without egg-yolk).In addition, methanol was tested as a cryoprotectant for B.
marinus sperm for the first by: We investigated the recovery of motility of cane toad (Bufo marinus) sperm after storage for 6 days at 0 degree C (on ice) and after subsequent cryopreservation.
Low temperature preservation. AbstractInvasive cane toads are colonizing southeastern Australia via a narrow coastal strip sandwiched between unsuitable areas (Pacific Ocean to the east, mountains to the west).
Many of the available spawning sites exhibit abiotic conditions (e.g., temperature, salinity, and pH) more extreme than those encountered elsewhere in the toad’s native or already invaded by: 4. Cryobiology. Aug;14(4) The low temperature preservation of boar spermatozoa.
The fertilizing capacity of frozen and thawed boar semen. recovery of motility and fertility of cryopreserved cane toad (Bufo masinus) sperm. Cryobiology. 37, - Bry, C., Temporal aspects of macroscopic changes in rainbow trout (Salmo gairdneri) oocytes before ovulation and of ova fertility during the post-ovulation period: effect of treatment with 17 alpha- hydroxybeta.
Past studies investigating the influence of abiotic factors on sperm performance during short term storage have shown that the temperature and osmolality of the storage solution can greatly affect. Temperature. Temperature is a key factor for the maturation of spermatozoa, and one of main reasons why in most mammals the testes are outside the body cavity (at least during the reproductive season).
A temperature of 37°C may be fatal for proper maturation and lead to infertility or even cause pathologies. MAININI has described (Semana Medica, 64,March ) a pregnancy test in which, following injection of pregnancy urine into the male toad (Bufo arenarum Hensel), liberation of spermatozoa.
On the other hand, refrigeration has been shown to be effective for maintaining motility over several days in cane toad (Bufo marinus) sperm collected from testes macerates (Browne et al., ) and from hormonally-induced spermatozoa of the dusky gopher frog (Rana sevosa), Fowler's toad (Bufo fowleri), Wyoming toad (Bufo baxteri), boreal toad.
The common toad was first given the name Rana bufo by the Swedish biologist Carl Linnaeus in the 10th edition of Systema Naturae in In this work, he placed all the frogs and toads in the single genus later became apparent that this genus should be divided, and inthe Austrian naturalist Josephus Nicolaus Laurenti placed the common toad in the genus Bufo, naming it Bufo : Amphibia.
Applied reproductive technologies and genetic resource banking for amphibian conservation Applied reproductive technologies and genetic resource banking for amphibian conservation Kouba Andrew J.
J.; Vance Carrie K. As amphibian populations continue to decline, both government and non-government organisations are establishing captive assurance. Barton HL, Guttman SI. Low temperature preservation of toad spermatozoa (Genus Bufo Pomering M, Clulow J. Storage of cane toad (Bufo marinus) sperm for 6 days at 0 deg C with subsequent cryopreservation.
Reprod Fertil Dev. d;– Amphibian Declines in the Twenty-First Century: Why We Need Assisted Reproductive Technologies Cited by: The effect of monosaccharides (glucose, fructose) and disaccharides (maltose, sucrose, trehalose) as diluents, in cryoprotective additives containing 15% (v/v) Me 2 SO or glycerol as cryoprotectants, were investigated on the recovery of sperm motility after cryopreservation of cane toad (Bufo marinus) spermatoazoa at low (5˚C min-1) and high.
Proc Soc Exp Biol Med. Apr;82(4) Observations on preservation of human spermatozoa at low temperatures. SHERMAN JK, BUNGE RG. In the U.S. alone, 55 of amphibian species, or about 21%, are threatened with extinction . One of these threatened species is the Wyoming toad (Bufo baxteri) with the last census revealing less than a hundred animals located in the wild .Cited by: Preservation of Washed Spermatozoa of Mehsana Buck at Refrigeration Temperature preservation in our study.
The increase in these 5. Islam, R., Ahmed, K. and Deka, B.C. Effect abnormalities mounted to ± and ± of holding and washing on the quality of goat semen. Accessory fibers in most sperm surround the axoneme so that their function in propulsion is difficult to assess.
In the sperm of the toad Bufo marinus, an accessory fiber is displaced from the axoneme, being connected to it by the thin undulating membrane in such a way that the movement of axoneme and accessory fiber can be viewed independently.Blood flow in systemic and pulmocutaneous arteries was measured as a function of body temperature (10°, 20°, and 30°C) at rest and following enforced physical activity in conscious, adult cane toads (Bufo marinus).Arterial and mixed venous hemoglobin concentration (C Hb) and total oxygen content (C o 2, tot) were measured in a separate group under identical by: Cryopreservation of Spermatozoa from Freeze-Intolerant Anurans The cryopreservation treatment judged most successful with spermatozoa of R.
sylvatica, Me2SO/FBS, was used to cryopreserve testicu-lar spermatozoa from R. pipiens (n 5 5) and B. americanus (n 5 6). Sperm lysis, sperm viabil-ity, viable sperm count, and recovery rate were.